Thromb Haemost 1982; 47(02): 182-184
DOI: 10.1055/s-0038-1657158
Original Article
Schattauer GmbH Stuttgart

Non-Decalcified Barium Sulfate-Adsorbed Plasma A Potentially Useful Reagent for Studying Blood Clotting, Platelets or Complement

Marjorie B Zucker
The Department of Pathology, New York University Medical Center, New York, NY and the Department of Medicine, Columbia College of Physicians and Surgeons, New York, NY, U.S.A.
,
John Owen
The Department of Pathology, New York University Medical Center, New York, NY and the Department of Medicine, Columbia College of Physicians and Surgeons, New York, NY, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 17 December 1981

Accepted 18 February 1982

Publication Date:
13 July 2018 (online)

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Summary

Plasma was prepared by rapid centrifugation of native blood followed by adsorption of vitamin K-dependent clotting factors with BaSO4. Compared to fresh citrated plasma, BaSO4-treated plasma contained about 80% of factor V and factor VIII and the same concentration of fibrinopeptide A. Assays were also carried out after overnight incubation of citrated plasma and BaSO4-adsorbed plasma with and without added citrate and compared to assays of fresh citrated plasma. Factor V decreased to about 25% in both citrated samples, factor VIII decreased to 45% in both samples of BaSO4-treated plasma, and fibrinopeptide A did not change. Thus loss of factor V activity depended on reduction in divalent cation concentration whereas loss of factor VIII activity may have resulted from effects of early traces of thrombin.